Article

Oct4 redox sensitivity potentiates reprogramming and differentiation

2023-02-21

Abstract excerpt

The transcription factor Oct4/Pou5f1 is a component of the regulatory circuitry governing pluripotency and is widely used to induce pluripotency from somatic cells. Here we use domain swapping and mutagenesis to study Oct4’s reprogramming ability, identifying a redox-sensitive DNA binding domain cysteine residue (Cys48) as a key determinant of reprogramming and differentiation. Oct4 Cys48 sensitizes the protein to...

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Literature Corpus work: fdfec0f6-b4b4-5ea4-ad34-4cad02a21145
DOI: 10.1101/2023.02.21.529404

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