Article

Highly Efficient Multiplexed Genome Engineering and Clone Selection to Enable Next Generation Induced Pluripotent Stem Cell (iPSC)-based Cell Therapies

2025-12-25

Abstract excerpt

Human induced pluripotent stem cells (iPSCs) have revolutionized regenerative medicine and cellular therapies. To improve the functionality and safety of iPSC-based therapies, genome engineering has been employed to disrupt gene expression and introduce therapeutic transgenes. However, current genome editing methods face significant challenges, including labor-intensive procedures, low efficiency, and safety conce...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: fa420075-2474-5375-940a-0fb243095722
DOI: 10.64898/2025.12.23.696205

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Highly Efficient Multiplexed Genome Engineering and Clone Selection to Enable Next Generation Induced Pluripotent Stem Cell (iPSC)-based Cell TherapiesDOI 10.64898/2025.12.23.696205Read

Genome editing in pluripotent stem cells: research and therapeutic applications.Semantic proximity Read

An iCRISPR platform for rapid, multiplexable, and inducible genome editing in human pluripotent stem cells.Semantic proximity Read

Combining Induced Pluripotent Stem Cells and Genome Editing Technologies for Clinical ApplicationsSemantic proximity Read

Genome Editing in Human Pluripotent Stem Cells: Approaches, Pitfalls, and Solutions.Semantic proximity Read

Multiplex genome editing of human pluripotent stem cells using Cpf1Semantic proximity Read

CRISPR Del/Rei: A simple, flexible, and efficient pipeline for scarless genome editingSemantic proximity Read

Efficient CRISPR-Cas9-mediated generation of knockin human pluripotent stem cells lacking undesired mutations at the targeted locus.Semantic proximity Read

Crispr-mediated Gene Targeting of Human Induced Pluripotent Stem Cells.Semantic proximity Read

Genome editing in induced pluripotent stem cellsSemantic proximity Read

Precision genome-editing with CRISPR/Cas9 in human induced pluripotent stem cellsSemantic proximity Read

A robust and inducible precise genome editing via an all-in-one prime editor in human pluripotent stem cellsSemantic proximity Read

Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesSemantic proximity Read