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Article

Crispr-mediated Gene Targeting of Human Induced Pluripotent Stem Cells.

Current protocols in stem cell biology - 1 Jan 2015

Byrne Susan M, Church George M

Abstract excerpt

CRISPR/Cas9 nuclease systems can create double-stranded DNA breaks at specific sequences to efficiently and precisely disrupt, excise, mutate, insert, or replace genes. However, human embryonic stem or induced pluripotent stem cells (iPSCs) are more difficult to transfect and less resilient to DNA damage than immortalized tumor cell lines. Here, we describe an optimized protocol for genome engineering of human...

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Identifiers

PMID: 26949444
DOI: 10.1002/9780470151808.sc05a08s35
PMCID: PMC4772967

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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