Article

CRISPR Del/Rei: A simple, flexible, and efficient pipeline for scarless genome editing

2021-01-18

Abstract excerpt

Scarless genome editing of induced pluripotent stem cells (iPSCs) is crucial for the precise modeling of genetic disease. Here we present CRISPR Del/Rei, a two-step deletion-reinsertion strategy with high editing efficiency and simple PCR-based screening that generates isogenic clones in ~2 months. We apply our strategy to edit iPSCs at 3 loci with only rare off target editing.

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Identifiers and source

Literature Corpus work: cebf4286-01f8-54bd-bad9-ea2852be7852
DOI: 10.1101/2021.01.18.427163

Open publication

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