Article
Efficient CRISPR-Cas9-mediated generation of knockin human pluripotent stem cells lacking undesired mutations at the targeted locus.
Cell reports - 12 May 2015
Merkle Florian T, Neuhausser Werner M, Santos David, Valen Eivind, Gagnon James A, Maas Kristi, Sandoe Jackson, Schier Alexander F, Eggan Kevin
Abstract excerpt
The CRISPR-Cas9 system has the potential to revolutionize genome editing in human pluripotent stem cells (hPSCs), but its advantages and pitfalls are still poorly understood. We systematically tested the ability of CRISPR-Cas9 to mediate reporter gene knockin at 16 distinct genomic sites in hPSCs. We observed efficient gene targeting but found that targeted clones carried an unexpectedly high frequency of...
Topics
- Base Sequence
- CRISPR-Cas Systems
- Clone Cells
- Gene Knock-In Techniques
- Gene Targeting
- Genetic Loci
- Humans
- INDEL Mutation
- Molecular Sequence Data
- Mutation