Article

Make-or-break prime editing for bacterial genome engineering

2024-06-28

Abstract excerpt

CRISPR-Cas9 has revolutionized genome engineering by allowing precise introductions of DNA double-strand breaks (DSBs). However, genome engineering in bacteria is still a complex, multi-step process requiring a donor DNA template for repair of DSBs. Prime editing circumvents this need as the repair template is indirectly provided within the prime editing guide RNA (pegRNA). Here, we developed make-or-break Prime E...

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Literature Corpus work: f7561431-45e5-5460-8016-d3bda103ca6e
DOI: 10.1101/2024.06.27.601116

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