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Improved nuclease-based prime editing by DNA repair modulation and pegRNA engineering

2024-02-02

Abstract excerpt

Prime editing is a genome engineering tool that allows installation of small edits with high precision. However, prime editing efficiency and purity can vary widely across different edits, genomic targets, and cell types. Prime editing nuclease (PEn) utilizes a fully active Cas9 instead of the nickase employed in conventional prime editors. PEn is capable of editing sites resistant to nickase-based prime editors b...

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Literature Corpus work
031ed6c7-2ede-5c04-ab5d-8b058b3f33fc
DOI
10.1101/2024.02.01.578377
Open publication

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Improved nuclease-based prime editing by DNA repair modulation and pegRNA engineeringDOI 10.1101/2024.02.01.578377
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