Improved Genome Editing by an Engineered CRISPR-Cas12a

Abstract excerpt

<h4>ABSTRACT</h4> CRISPR-Cas12a is an RNA-guided, programmable genome editing enzyme found within bacterial adaptive immune pathways. Unlike CRISPR-Cas9, Cas12a uses only a single catalytic site to both cleave target double-stranded DNA (dsDNA) ( cis -activity) and indiscriminately degrade single-stranded DNA (ssDNA) ( trans -activity). To investigate how the relative potency of cis - versus trans -DNase acti...

Topics

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• Bacteria
• Clustered Regularly Interspaced Short Palindromic Repeats
• CRISPR and Genetic Engineering
• CRISPR-Cas Systems
• DNA Repair
• Genetic Engineering
• Genome, Bacterial
• Insect symbiosis and bacterial influences

Identifiers and source

Literature Corpus work

8c34b07c-9879-5a7b-8036-e75013a2c02b

DOI

10.1101/2022.09.02.506401