Article

Targeted DNA integration in human cells without double-strand breaks using CRISPR RNA-guided transposases

2023-03-18

Abstract excerpt

Traditional genome-editing reagents such as CRISPR-Cas9 achieve targeted DNA modification by introducing double-strand breaks (DSBs), thereby stimulating localized DNA repair by endogenous cellular repair factors. While highly effective at generating heterogenous knockout mutations, this approach suffers from undesirable byproducts and an inability to control product purity. Here we develop a system in human cells...

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Identifiers and source

Literature Corpus work: 5b4bb8ea-f428-5393-bb21-1116ca5884b5
DOI: 10.1101/2023.03.17.533036

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