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Improved genome editing by an engineered CRISPR-Cas12a

29 Nov 2022

Abstract excerpt

CRISPR-Cas12a is an RNA-guided, programmable genome editing enzyme found within bacterial adaptive immune pathways. Unlike CRISPR-Cas9, Cas12a uses only a single catalytic site to both cleave target double-stranded DNA (dsDNA) (cis-activity) and indiscriminately degrade single-stranded DNA (ssDNA) (trans-activity). To investigate how the relative potency of cis- versus trans-DNase activity affects Cas12a-mediated...

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PMID: 36537251
DOI: 10.1093/nar/gkac1192

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