Article

Drag-and-drop genome insertion without DNA cleavage with CRISPR-directed integrases

2021-11-01

Abstract excerpt

Programmable and multiplexed genome integration of large, diverse DNA cargo independent of DNA repair remains an unsolved challenge of genome editing. Current gene integration approaches require double-strand breaks that evoke DNA damage responses and rely on repair pathways that are inactive in terminally differentiated cells. Furthermore, CRISPR-based approaches that bypass double stranded breaks, such as Prime...

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Literature Corpus work: 5c69db9a-b42e-516f-be5d-a03705407fb1
DOI: 10.1101/2021.11.01.466786

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