Article
A versatile and highly efficient method for scarless genome editing in Escherichia coli and Salmonella enterica.
BMC biotechnology - 25 Sept 2014
Kim Juhan, Webb Anthony M, Kershner Jamie P, Blaskowski Stephen, Copley Shelley D
Abstract excerpt
BACKGROUND: Recently developed methods for genome editing in bacteria take advantage of the introduction of double-strand breaks by I-SceI in a mutation cassette to select for cells in which homologous recombination has healed the break and introduced a desired mutation. This elegantly designed method did not work well in our hands for most genes. RESULTS: We corrected a mutation in the gene encoding I-SceI that...
Topics
- Escherichia coli
- Genes, Essential
- Genome, Bacterial
- Mutagenesis, Insertional
- Mutation
- Plasmids
- Salmonella enterica