Efficient Genome Editing with Chimeric Oligonucleotide-Directed Editing

Abstract excerpt

Prime editing has emerged as a precise and powerful genome editing tool, offering a favorable gene editing profile compared to other Cas9-based approaches. Here we report new nCas9-DNA polymerase fusion proteins to create chimeric oligonucleotide-directed editing (CODE) systems for search-and-replace genome editing. Through successive rounds of engineering, we developed CODEMax and CODEMax(exo+) editors that achie...

Topics

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• CRISPR-Associated Protein 9
• CRISPR-Cas Systems
• DNA Mismatch Repair
• Endonucleases
• Gene Editing
• Genes, Dominant
• HEK293 Cells
• Mutation

Identifiers and source

Literature Corpus work

3615d4e0-c529-5e7b-b429-c24d26a54ae4

DOI

10.1101/2024.07.09.602710