Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases.

doi:10.1101/gr.162339.113

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Article

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases.

Genome research - 1 Jan 2014

Cho Seung Woo, Kim Sojung, Kim Yongsub, Kweon Jiyeon, Kim Heon Seok, Bae Sangsu, Kim Jin-Soo

Abstract excerpt

RNA-guided endonucleases (RGENs), derived from the prokaryotic adaptive immune system known as CRISPR/Cas, enable targeted genome engineering in cells and organisms. RGENs are ribonucleoproteins that consist of guide RNA and Cas9, a protein component originated from Streptococcus pyogenes. These enzymes cleave chromosomal DNA, whose sequence is complementary, to guide RNA in a targeted manner, producing...

Topics

Base Sequence
Binding Sites
CRISPR-Associated Proteins
CRISPR-Cas Systems
Chromosomes, Human
DNA
DNA Breaks, Double-Stranded
Deoxyribonuclease I
Endonucleases
Exome
Genetic Loci

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genome, Human

HeLa Cells

High-Throughput Nucleotide Sequencing

Humans

K562 Cells

Models, Genetic

Mutation

Source

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Identifiers

PMID: 24253446
DOI: 10.1101/gr.162339.113
PMCID: PMC3875854

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-24253446-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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