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Article

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases.

Genome research - 1 Jan 2014

Cho Seung Woo, Kim Sojung, Kim Yongsub, Kweon Jiyeon, Kim Heon Seok, Bae Sangsu, Kim Jin-Soo

Abstract excerpt

RNA-guided endonucleases (RGENs), derived from the prokaryotic adaptive immune system known as CRISPR/Cas, enable targeted genome engineering in cells and organisms. RGENs are ribonucleoproteins that consist of guide RNA and Cas9, a protein component originated from Streptococcus pyogenes. These enzymes cleave chromosomal DNA, whose sequence is complementary, to guide RNA in a targeted manner, producing...

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PMID: 24253446
DOI: 10.1101/gr.162339.113
PMCID: PMC3875854

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