Article

A step by step protocol for genomic knock-in of long sequences using dCas9-SSAP editor

2022-05-19

Abstract excerpt

<title>Abstract</title> <p>Gene-editing is a powerful tool for probing the mechanisms of human health and diseases, and holds promise as a durable therapeutic approach. Exemplified by CRISPR-Cas systems, gene-editing tools often cause DNA damage at on- and off-target sites, and thus trigger diverse endogenous DNA repair processes that are error-prone. Such unwanted mutations and safety concerns can be exacerbated...

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Literature Corpus work: 4a6d16e6-dab9-5ad0-b0c0-34bfbe0a4de4
DOI: 10.21203/rs.3.pex-1711/v1

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