Article

Detection of precisely edited CRISPR/Cas9 alleles through co-introduced restriction-fragment length polymorphisms

2021-04-20

Abstract excerpt

<h4>ABSTRACT</h4> CRISPR/Cas9 is a powerful tool for producing genomic in sertions and del etions (indels) to interrogate gene function. Modified CRISPR/Cas9 protocols can produce targeted genetic changes that are more precise than indels, but founder recovery is less efficient. Focusing on producing missense mutations in zebrafish using s ingle- s tranded o ligo d eoxy n ucleotide (ssODN) donor templates,...

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Literature Corpus work: ee9e9695-ab0c-5e68-867d-87c0a3f4d624
DOI: 10.1101/2021.04.20.440537

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