Article
Efficient identification of CRISPR/Cas9-induced insertions/deletions by direct germline screening in zebrafish.
BMC genomics - 24 Mar 2016
Brocal Isabel, White Richard J, Dooley Christopher M, Carruthers Samantha N, Clark Richard, Hall Amanda, Busch-Nentwich Elisabeth M, Stemple Derek L, Kettleborough Ross N W
Abstract excerpt
BACKGROUND: The CRISPR/Cas9 system is a prokaryotic immune system that infers resistance to foreign genetic material and is a sort of 'adaptive immunity'. It has been adapted to enable high throughput genome editing and has revolutionised the generation of targeted mutations. RESULTS: We have developed a scalable analysis pipeline to identify CRISPR/Cas9 induced mutations in hundreds of samples using next...
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