Article
High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Genome research - 1 Jul 2015
Varshney Gaurav K, Pei Wuhong, LaFave Matthew C, Idol Jennifer, Xu Lisha, Gallardo Viviana, Carrington Blake, Bishop Kevin, Jones MaryPat, Li Mingyu, Harper Ursula, Huang Sunny C, Prakash Anupam, Chen Wenbiao, Sood Raman, Ledin Johan, Burgess Shawn M
Abstract excerpt
The use of CRISPR/Cas9 as a genome-editing tool in various model organisms has radically changed targeted mutagenesis. Here, we present a high-throughput targeted mutagenesis pipeline using CRISPR/Cas9 technology in zebrafish that will make possible both saturation mutagenesis of the genome and large-scale phenotyping efforts. We describe a cloning-free single-guide RNA (sgRNA) synthesis, coupled with streamlined...
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