Establishment of the CRISPR/Cas9 System for Targeted Gene Disruption and Gene Tagging.

doi:10.1007/978-1-4939-7231-9_11

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Article

Establishment of the CRISPR/Cas9 System for Targeted Gene Disruption and Gene Tagging.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017

Ehrke-Schulz Eric, Schiwon Maren, Hagedorn Claudia, Ehrhardt Anja

Abstract excerpt

CRISPR/Cas9 RNA-guided nucleases refashioned in vivo gene editing approaches for specific gene disruption, gene correction, or gene addition. Moreover, chimeric Cas9 proteins can be applied to direct fused cis-acting effector protein domains, enzymes, or fluorescent markers to DNA to target seque...

Topics

CRISPR-Cas Systems
DNA
Mutation
RNA Editing
RNA, Guide, CRISPR-Cas Systems

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