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Article

Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs.

PloS one - 1 Jan 2014

Gagnon James A, Valen Eivind, Thyme Summer B, Huang Peng, Akhmetova Laila, Ahkmetova Laila, Pauli Andrea, Montague Tessa G, Zimmerman Steven, Richter Constance, Schier Alexander F

Abstract excerpt

The CRISPR/Cas9 system has been implemented in a variety of model organisms to mediate site-directed mutagenesis. A wide range of mutation rates has been reported, but at a limited number of genomic target sites. To uncover the rules that govern effective Cas9-mediated mutagenesis in zebrafish, we targeted over a hundred genomic loci for mutagenesis using a streamlined and cloning-free method. We generated...

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Identifiers

PMID: 24873830
DOI: 10.1371/journal.pone.0098186
PMCID: PMC4038517

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Source receipt: pubmed, retrieved 7 Sept 2026.

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