Article
CRISPR-Cas9 induces large structural variants at on-target and off-target sites in vivo that segregate across generations.
Nature communications - 2 Feb 2022
Höijer Ida, Emmanouilidou Anastasia, Östlund Rebecka, van Schendel Robin, Bozorgpana Selma, Tijsterman Marcel, Feuk Lars, Gyllensten Ulf, den Hoed Marcel, Ameur Adam
Abstract excerpt
CRISPR-Cas9 genome editing has potential to cure diseases without current treatments, but therapies must be safe. Here we show that CRISPR-Cas9 editing can introduce unintended mutations in vivo, which are passed on to the next generation. By editing fertilized zebrafish eggs using four guide RNAs selected for off-target activity in vitro, followed by long-read sequencing of DNA from >1100 larvae, juvenile and...
Topics
- Animals
- CRISPR-Cas Systems
- DNA
- Gene Editing
- Genetic Therapy
- Germ Cells
- Humans
- Mutation
- RNA, Guide, CRISPR-Cas Systems
- Zebrafish