Article

CRISPR-Cas9 induces large structural variants at on-target and off-target sites <i>in vivo</i> that segregate across generations

2021-10-05

Abstract excerpt

To investigate the extent and distribution of unintended mutations induced by CRISPR-Cas9 in vivo, we edited the genome of fertilized zebrafish eggs and investigated DNA from >1100 larvae, juvenile and adult fish in the F0 and F1 generations. Four guide RNAs (gRNAs) were used, selected from 23 gRNAs with high on-target efficiency in vivo in previous functional experiments. CRISPR-Cas9 outcomes were analyzed by l...

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Literature Corpus work: 1a848ad8-fbed-53c0-8dec-9bfb8c59a70c
DOI: 10.1101/2021.10.05.463186

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