Article

Successful optimization of CRISPR/Cas9-mediated defined point mutation knock-in using allele-specific PCR assays in zebrafish

2017-09-27

Abstract excerpt

Single-stranded oligodeoxynucleotides (ssODN) are donor templates for homology-directed repair-based knock-in of point mutations using CRISPR/Cas9. To optimize the efficiency of ssODN-based knock-ins in zebrafish, we developed allele-specific PCR (AS-PCR) assays for introducing point mutations in tp53 , cdh5 and lmna as case studies. In these point mutation strategies we introduced the codon mutations, sgRNA si...

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Literature Corpus work: 680ae6a9-f421-5b74-b59b-2297ae746b52
DOI: 10.1101/194936

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