Article

Effective <i>in vivo</i> binding energy landscape illustrates kinetic stability of RBPJ-DNA binding

2023-12-20

Abstract excerpt

Transcription factors (TFs) such as the central DNA-binding hub in Notch signal transduction, RBPJ, bind to specific DNA sequences to regulate gene transcription. How the efficiency of gene regulation depends on the TF-DNA binding kinetics and cofactor interactions is mostly unknown. We determined the DNA binding kinetics and the transcriptional activity of RBPJ and several mutant variants by live-cell single-mole...

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Literature Corpus work: dc964efd-ce89-5d14-9253-42dd365861ec
DOI: 10.1101/2023.12.19.572376

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