Article
Context-Dependent Gene Regulation by Homeodomain Transcription Factor Complexes Revealed by Shape-Readout Deficient Proteins.
Molecular cell - 2 Apr 2020
Kribelbauer Judith F, Loker Ryan E, Feng Siqian, Rastogi Chaitanya, Abe Namiko, Rube H Tomas, Bussemaker Harmen J, Mann Richard S
Abstract excerpt
Eukaryotic transcription factors (TFs) form complexes with various partner proteins to recognize their genomic target sites. Yet, how the DNA sequence determines which TF complex forms at any given site is poorly understood. Here, we demonstrate that high-throughput in vitro DNA binding assays coupled with unbiased computational analysis provide unprecedented insight into how different DNA sequences select...
Topics
- Animals
- Base Sequence
- Binding Sites
- DNA
- Drosophila Proteins
- Drosophila melanogaster
- Gene Expression Regulation
- Homeodomain Proteins
- Mutation
- Nucleic Acid Conformation