Article
Single-molecule imaging of SWI/SNF chromatin remodelers reveals bromodomain-mediated and cancer-mutants-specific landscape of multi-modal DNA-binding dynamics.
Nature communications - 2 Sept 2024
Engl Wilfried, Kunstar-Thomas Aliz, Chen Siyi, Ng Woei Shyuan, Sielaff Hendrik, Zhao Ziqing Winston
Abstract excerpt
Despite their prevalent cancer implications, the in vivo dynamics of SWI/SNF chromatin remodelers and how misregulation of such dynamics underpins cancer remain poorly understood. Using live-cell single-molecule tracking, we quantify the intranuclear diffusion and chromatin-binding of three key subunits common to all major human SWI/SNF remodeler complexes (BAF57, BAF155 and BRG1), and resolve two temporally...
Topics
- Humans
- Transcription Factors
- Single Molecule Imaging
- Nuclear Proteins
- DNA Helicases
- Chromosomal Proteins, Non-Histone
- Chromatin Assembly and Disassembly
- Chromatin
- Neoplasms
- DNA
- DNA-Binding Proteins
- Protein Binding
- Mutation