Article

Pythia: Non-random DNA repair allows predictable CRISPR/Cas9 integration and gene editing

2024-09-23

Abstract excerpt

<h4>Summary</h4> CRISPR-based genome engineering holds enormous promise for basic science and therapeutic applications. Integrating and editing DNA sequences is still challenging in many cellular contexts, largely due to insufficient control of the repair process. We find that repair at the genome-cargo interface is predictable by deep-learning models and adheres to sequence context specific rules. Based on in s...

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Literature Corpus work: d5d3786a-280b-5d83-a224-435133726c1e
DOI: 10.1101/2024.09.23.614424

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