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<i>In cellulo</i> DNA assembly for targeted genomic integration and rearrangement in human cells

2025-06-16

Abstract excerpt

Although therapeutic genome editing holds great potential to remedy diverse inherited and acquired disorders, targeted installation of medium to large sized genomic modifications in therapeutically relevant cells remains challenging. We have developed an approach that permits DNA sequence assembly and integration in human cells leveraging CRISPR-targeted dual flap synthesis. This method, named prime assembly, allo...

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Identifiers and source

Literature Corpus work
075ca482-5822-5413-8bdf-05884fcd3259
DOI
10.1101/2025.06.16.659926
Open publication

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<i>In cellulo</i> DNA assembly for targeted genomic integration and rearrangement in human cellsDOI 10.1101/2025.06.16.659926
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