Article

Enabling large-scale genome editing by reducing DNA nicking

2019-03-15

Abstract excerpt

To extend the frontier of genome editing and enable the radical redesign of mammalian genomes, we developed a set of dead-Cas9 base editor (dBE) variants that allow editing at tens of thousands of loci per cell by overcoming the cell death associated with DNA double-strand breaks (DSBs) and single-strand breaks (SSBs). We used a set of gRNAs targeting repetitive elements – ranging in target copy number from about...

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Literature Corpus work: d6d49d1e-aa5a-529b-9bf2-18e5a809ef1c
DOI: 10.1101/574020

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