Article

A Homology Independent Sequence Replacement Strategy in Human Cells Using a CRISPR Nuclease

2020-05-12

Abstract excerpt

Precision genomic alterations largely rely on Homology Directed Repair (HDR), but targeting without homology using the Non-Homologous End Joining (NHEJ) pathway has gained attention as a promising alternative. Previous studies demonstrated precise insertions formed by the ligation of donor DNA into a targeted genomic double strand break in both dividing and non-dividing cells. Here we extend this idea and use NHEJ...

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Literature Corpus work: 8079dfc3-c706-5dd5-ac1e-7e11c53ff355
DOI: 10.1101/2020.05.11.088252

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