Article
The interplay of DNA repair context with target sequence predictably biases Cas9-generated mutations.
Nature communications - 27 Nov 2024
Pallaseni Ananth, Peets Elin Madli, Girling Gareth, Crepaldi Luca, Kuzmin Ivan, Moor Marilin, Muñoz-Subirana Núria, Schimmel Joost, Serçin Özdemirhan, Mardin Balca R, Tijsterman Marcel, Peterson Hedi, Kosicki Michael, Parts Leopold
Abstract excerpt
Repair of double-stranded breaks generated by CRISPR/Cas9 is highly dependent on the flanking DNA sequence. To learn about interactions between DNA repair and target sequence, we measure frequencies of over 236,000 distinct Cas9-generated mutational outcomes at over 2800 synthetic target sequences in 18 DNA repair deficient mouse embryonic stem cells lines. We classify the outcomes in an unbiased way, finding a...
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