Article

Massively parallel profiling and predictive modeling of the outcomes of CRISPR/Cas9-mediated double-strand break repair

2018-11-28

Abstract excerpt

Non-homologous end-joining (NHEJ) plays an important role in double-strand break (DSB) repair of DNA. Recent studies have shown that the error patterns of NHEJ are strongly biased by sequence context, but these studies were based on relatively few templates. To investigate this more thoroughly, we systematically profiled ∼1.16 million independent mutational events resulting from CRISPR/Cas9-mediated cleavage and N...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 69448c51-6422-5594-b384-aab2dca34d90
DOI: 10.1101/481069

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Massively parallel profiling and predictive modeling of the outcomes of CRISPR/Cas9-mediated double-strand break repairDOI 10.1101/481069Read

Global detection of DNA repair outcomes induced by CRISPR-Cas9Semantic proximity Read

A comprehensive genetic catalog of human double-strand break repairSemantic proximity Read

Target-specific precision of CRISPR-mediated genome editingSemantic proximity Read

Predicting Mutations Generated by Cas9, Base Editing, and Prime Editing in Mammalian Cells.Semantic proximity Read

The interplay of DNA repair context with target sequence predictably biases Cas9-generated mutations.Semantic proximity Read

Decoding non-random mutational signatures at Cas9 targeted sites.Semantic proximity Read

Mapping the genetic landscape of DNA double-strand break repair.Semantic proximity Read

Mutational signature catalogue <i>(MUSIC)</i> of DNA double-strand break repairSemantic proximity Read

Systematic characterization of genome editing in primary T cells reveals proximal genomic insertions and enables machine learning prediction of CRISPR-Cas9 DNA repair outcomesSemantic proximity Read

Robust activation of microhomology-mediated end joining for precision gene editing applications.Semantic proximity Read

Developmental progression of DNA double-strand break repair deciphered by a single-allele resolution mutation classifier.Semantic proximity Read

Quantitative analysis shows that repair of Cas9-induced double-strand DNA breaks is slow and error-proneSemantic proximity Read