Article

Repair of CRISPR-guided RNA breaks enables site-specific RNA editing in human cells

2023-08-29

Abstract excerpt

Genome editing with CRISPR RNA-guided endonucleases generates DNA breaks that are resolved by cellular DNA repair machinery. However, analogous methods to manipulate RNA remain unavailable. Here, we show that site-specific RNA breaks generated with RNA-targeting CRISPR complexes are repaired in human cells, and this repair can be used for programmable deletions in human transcripts that restore gene function. Coll...

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Literature Corpus work: 1e83c8e2-eb84-5e00-8489-ae6cd327ab88
DOI: 10.1101/2023.08.29.555404

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