Article

Structure-optimized sgRNA selection with PlatinumCRISPr for efficient Cas9 generation of knock-outs

2022-12-15

Abstract excerpt

A single guide RNA (sgRNA) directs Cas9 nuclease for gene-specific scission of double-stranded DNA. High Cas9 activity is essential for efficient gene editing to generate gene deletions and gene replacements by homologous recombination. However, cleavage efficiency is below 50% for more than half of randomly selected sgRNA sequences in human cell culture screens or model organisms. We used in vitro assays to deter...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 0b9a984b-dc14-5947-953f-1954f57a0ac7
DOI: 10.1101/2022.12.15.520550

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Structure-optimized sgRNA selection with PlatinumCRISPr for efficient Cas9 generation of knock-outsDOI 10.1101/2022.12.15.520550Read

Principles for rational Cas13d guide designSemantic proximity Read

CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo.Semantic proximity Read

Enhanced specificity and efficiency of the CRISPR/Cas9 system with optimized sgRNA parameters in Drosophila.Semantic proximity Read

Scalable Design of paired CRISPR Guide RNAs for Genomic DeletionSemantic proximity Read

Pythia: Non-random DNA repair allows predictable CRISPR/Cas9 integration and gene editingSemantic proximity Read

A benchmark comparison of CRISPRn guide-RNA design algorithms and generation of small single and dual-targeting libraries to boost screening efficiencySemantic proximity Read

CasFinder: Flexible algorithm for identifying specific Cas9 targets in genomesSemantic proximity Read

High-throughput evaluation of in vitro CRISPR activities enables optimized large-scale multiplex enrichment of rare variants.Semantic proximity Read

Partial DNA-guided Cas9 enables genome editing with reduced off-target activity.Semantic proximity Read

protoSpaceJAM: an open-source, customizable and web-accessible design platform for CRISPR/Cas insertional knock-in.Semantic proximity Read

Massively Parallel Profiling of RNA-targeting CRISPR-Cas13dSemantic proximity Read

GUIDES: sgRNA design for loss-of-function screensSemantic proximity Read