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Cohort-stratified prioritization of CRISPR–Cas9 sgRNAs for HDR-mediated correction of <i>TP53</i> hotspot codons in ovarian, pancreatic, and colorectal cancer

2026-05-22

Abstract excerpt

TP53 is mutated in roughly half of all human cancers. Eight recurrent missense substitutions in the DNA-binding domain (R175H, Y220C, G245S, R248Q, R248W, R249S, R273H, R282W) account for most of the mutational burden. Homology-directed repair (HDR) with a wild-type donor template is one of the few feasible routes to revert these alleles, but existing CRISPR sgRNA design tools rank candidates without reference to...

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Identifiers and source

Literature Corpus work
d45b4811-e814-54b4-b3f8-c5efa593901d
DOI
10.64898/2026.05.20.726726
Open publication

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