Article
Synthetic mismatches enable specific CRISPR-Cas12a-based detection of genome-wide SNVs tracked by ARTEMIS.
Cell reports methods - 16 Dec 2024
Kohabir Kavish A V, Linthorst Jasper, Nooi Lars O, Brouwer Rick, Wolthuis Rob M F, Sistermans Erik A
Abstract excerpt
Detection of pathogenic DNA variants is vital in cancer diagnostics and treatment monitoring. While CRISPR-based diagnostics (CRISPRdx) offer promising avenues for cost-effective, rapid, and point-of-care testing, achieving single-nucleotide detection fidelity remains challenging. We present an in silico pipeline that scans the human genome for targeting pathogenic mutations in the seed region (ARTEMIS), the most...
Topics
- Humans
- CRISPR-Cas Systems
- Proto-Oncogene Proteins B-raf
- Aldehyde Dehydrogenase, Mitochondrial
- Genome, Human
- Polymorphism, Single Nucleotide
- Endodeoxyribonucleases
- CRISPR-Associated Proteins
- BRCA2 Protein
- Neoplasms