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Article

A Modular and Programmable Cas13d Platform for RNA Single Nucleotide Variant Detection.

Advanced science (Weinheim, Baden-Wurttemberg, Germany) - 1 Jun 2026

Wang Zeyu, Li Jiahao, Yue Zhuying, He Beixuan, Zhang Wenhua, Fang Zhen, Xia Qing, Liu Yingbin, Li Yanjing

Abstract excerpt

CRISPR-based nucleic acid diagnostics have shown broad potential, yet reliable single-nucleotide variant (SNV) discrimination remains limited by flanking sequence requirements that constrain targetability, and an inherent specificity-sensitivity trade-off where mismatch designs used to suppress wild type recognition often penalize enzymatic activity. Here we develop a scenario-guided Cas13d framework that...

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Identifiers

PMID: 42080370
DOI: 10.1002/advs.202523680
PMCID: PMC13252642

Genes

IDH1
BRAF
KRAS

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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