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Article

In Vitro CRISPR-Cas12a-Based Detection of Cancer-Associated TP53 Hotspot Mutations Beyond the crRNA Seed Region.

The CRISPR journal - 1 Apr 2023

Kohabir Kavish A V, Nooi Lars O, Brink Arjen, Brakenhoff Ruud H, Sistermans Erik A, Wolthuis Rob M F

Abstract excerpt

Cost-effective and time-efficient detection of oncogenic mutations supports improved presymptomatic cancer diagnostics and post-treatment disease monitoring. Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12a is an RNA-guided endonuclease that, upon protospacer adjacent motif (PAM)-dependent recognition of target DNA in cis, exhibits indiscriminate ssDNase activity in trans, which can be...

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Identifiers

PMID: 36637878
DOI: 10.1089/crispr.2022.0077
PMCID: PMC10123810

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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