Article
Deep CRISPR mutagenesis characterizes the functional diversity of TP53 mutations.
Nature genetics - 1 Jan 2025
Funk Julianne S, Klimovich Maria, Drangenstein Daniel, Pielhoop Ole, Hunold Pascal, Borowek Anna, Noeparast Maxim, Pavlakis Evangelos, Neumann Michelle, Balourdas Dimitrios-Ilias, Kochhan Katharina, Merle Nastasja, Bullwinkel Imke, Wanzel Michael, Elmshäuser Sabrina, Teply-Szymanski Julia, Nist Andrea, Procida Tara, Bartkuhn Marek, Humpert Katharina, Mernberger Marco, Savai Rajkumar, Soussi Thierry, Joerger Andreas C, Stiewe Thorsten
Abstract excerpt
The mutational landscape of TP53, a tumor suppressor mutated in about half of all cancers, includes over 2,000 known missense mutations. To fully leverage TP53 mutation status for personalized medicine, a thorough understanding of the functional diversity of these mutations is essential. We conducted a deep mutational scan using saturation genome editing with CRISPR-mediated homology-directed repair to engineer...
Topics
- Humans
- Tumor Suppressor Protein p53
- CRISPR-Cas Systems
- Gene Editing
- Neoplasms
- Cell Line, Tumor
- Mutagenesis
- Mutation
- Mutation, Missense