Article

Benchmarking of SpCas9 variants enables deeper base editor screens of <i>BRCA1</i> and <i>BCL2</i>

2021-08-18

Abstract excerpt

<h4>ABSTRACT</h4> Numerous rationally-designed and directed-evolution variants of SpCas9 have been reported to expand the utility of CRISPR technology. Here, we benchmark PAM preferences, on-target activity, and off-target susceptibility of 11 variants of SpCas9 in cell culture assays with thousands of guides targeting endogenous genes. To enhance the coverage and thus utility of base editing screens, we demonstr...

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Literature Corpus work: dbb202d0-ad56-5bd5-ae3e-e68f15f0ece8
DOI: 10.1101/2021.08.18.456848

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