Article

An efficient and adaptable workflow for editing disease-relevant single nucleotide variants using CRISPR/Cas9

2021-11-12

Abstract excerpt

Single nucleotide variants are the commonest genetic alterations in the human genome. At least 60,000 have been reported to be associated with disease. The CRISPR/Cas9 system has transformed genetic research, making it possible to edit single nucleotides and study the function of genetic variants in vitro . While significant advances have improved the efficiency of CRISPR/Cas9, the editing of single nucleotides r...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 88658aee-dd49-54b2-8a98-4a66356f03f6
DOI: 10.1101/2021.11.12.467071

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

An efficient and adaptable workflow for editing disease-relevant single nucleotide variants using CRISPR/Cas9DOI 10.1101/2021.11.12.467071Read

Activity-based selection for enhanced base editor mutational scanningSemantic proximity Read

Atlas of CRISPR Correction of Pathogenic Human Genetic VariantsSemantic proximity Read

Exploring the Efficiency of Multi-Target Editing with Single Base Editors in Haematopoietic Cell linesSemantic proximity Read

Precise and efficient scarless genome editing in stem cells using CORRECT.Semantic proximity Read

P53 toxicity is a hurdle to CRISPR/CAS9 screening and engineering in human pluripotent stem cellsSemantic proximity Read

In the business of base editors: Evolution from bench to bedsideSemantic proximity Read

Precision genome editing using cytosine and adenine base editors in mammalian cells.Semantic proximity Read

SNIPSNP: precision design of CRISPR/Cas9 knock-in reagents for variant correction and disease modeling.Semantic proximity Read

Protocol for Non-viral HDR-based CRISPR/Cas9 platform for small custom editing in primary T cellsSemantic proximity Read

Genome scale analysis of pathogenic variants targetable for single base editing.Semantic proximity Read

Programmable multi-kilobase RNA editing using CRISPR-mediated trans-splicingSemantic proximity Read

Therapeutic Editing of the TP53 Gene: Is CRISPR/Cas9 an Option?Semantic proximity Read