Article

High-resolution genome-wide mapping of chromosome-arm-scale truncations induced by CRISPR-Cas9 editing

2023-04-15

Abstract excerpt

<h4>Summary</h4> CRISPR-Cas9 editing is a scalable technology for mapping of biological pathways, but it has been reported to cause a variety of undesired large-scale structural changes to the genome. We performed an arrayed CRISPR-Cas9 scan of the genome in primary human cells, targeting 17,065 genes for knockout with 101,029 guides. High-dimensional phenomics reveals a “proximity bias” in which CRISPR knockouts...

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Literature Corpus work: cdeba6d4-1eae-5e6a-8c6a-3da0ceb14809
DOI: 10.1101/2023.04.15.537038

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