Article

Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-Seq+

2022-10-30

Abstract excerpt

<h4>ABSTRACT</h4> Discovery of off-target CRISPR-Cas genome editing activity in patient-derived cells and animal models is crucial for therapeutic applications, but currently exhibits low sensitivity. We demonstrate that inhibition of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) accumulates repair protein MRE11 at CRISPR-targeted sites, enabling high-sensitivity mapping of off-target sites to positio...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: a4689840-4a01-558b-9694-8896b763b2dd
DOI: 10.1101/2022.10.29.514376

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-Seq+DOI 10.1101/2022.10.29.514376Read

Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-Seq+Semantic proximity Read

Paired CRISPR screens to map gene regulation in <i>cis</i> and <i>trans</i>Semantic proximity Read

High-resolution genome-wide mapping of chromosome-arm-scale truncations induced by CRISPR-Cas9 editingSemantic proximity Read

Massively Targeted Evaluation of Therapeutic CRISPR Off-Targets in CellsSemantic proximity Read

Applications of CRISPR-Cas9 for advancing precision medicine in oncology: from target discovery to disease modelingSemantic proximity Read

Precision CRISPR annotation of the functional enhancer landscape in primary human T cellsSemantic proximity Read

crisprQTL mapping as a genome-wide association framework for cellular genetic screensSemantic proximity Read

CRISPR-Cas9-mediated genome engineering exaggerates genomic deletion at 10q23.31 including the <i>PTEN</i> gene locus mimicking cancer profilesSemantic proximity Read

Coupled single-cell CRISPR screening and epigenomic profiling reveals causal gene regulatory networksSemantic proximity Read

CHANGE-seq reveals genetic and epigenetic effects on CRISPR-Cas9 genome-wide activity.Semantic proximity Read

Programmable CRISPRtune dissects the transcriptional repressive activity of MeCP2Semantic proximity Read

CasFinder: Flexible algorithm for identifying specific Cas9 targets in genomesSemantic proximity Read