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A CRISPR-del-based pipeline for complete gene knockout in human diploid cells

2021-06-22

Abstract excerpt

The advance of CRISPR/Cas9 technology has enabled us easily to generate gene knockout cell lines by introducing insertion/deletion mutations (indels) at the target site via the error-prone non-homologous end joining repair system. Frameshift-promoting indels can disrupt gene functions by generation of a premature stop codon. However, there is growing evidence that targeted genes are not always knocked-out by the i...

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Identifiers and source

Literature Corpus work
d224e149-56b8-53f3-936f-0042cc290114
DOI
10.1101/2021.06.21.449335
Open publication

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A CRISPR-del-based pipeline for complete gene knockout in human diploid cellsDOI 10.1101/2021.06.21.449335
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