Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Intrinsic deletion at 10q23.31, including the PTEN gene locus, is aggravated upon CRISPR-Cas9-mediated genome engineering in HAP1 cells mimicking cancer profiles.

Life science alliance - 1 Feb 2024

Geng Keyi, Merino Lara G, Veiga Raül G, Sommerauer Christian, Epperlein Janine, Brinkman Eva K, Kutter Claudia

Abstract excerpt

The CRISPR-Cas9 system is a powerful tool for studying gene functions and holds potential for disease treatment. However, precise genome editing requires thorough assessments to minimize unintended on- and off-target effects. Here, we report an unexpected 283-kb deletion on Chromosome 10 (10q23.31) in chronic myelogenous leukemia-derived HAP1 cells, which are frequently used in CRISPR screens. The deleted region...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CRISPR-Cas Systems
Share to Gene Editing

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genome

Share to Neoplasms

Share to Phenotype

Share to PTEN Phosphohydrolase

Source

Open in PubMed Open full text

Identifiers

PMID: 37984988
DOI: 10.26508/lsa.202302128
PMCID: PMC10662290

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Intrinsic deletion at 10q23.31, including the PTEN gene locus, is aggravated upon CRISPR-Cas9-mediated genome engineering in HAP1 cells mimicking cancer profiles.PMID 37984988Read ↗

CRISPR-Cas9-mediated genome engineering exaggerates genomic deletion at 10q23.31 including the <i>PTEN</i> gene locus mimicking cancer profilesSemantic proximity Read ↗

High-resolution genome-wide mapping of chromosome-arm-scale truncations induced by CRISPR-Cas9 editingSemantic proximity Read ↗

Coupled single-cell CRISPR screening and epigenomic profiling reveals causal gene regulatory networksSemantic proximity Read ↗

Genome-wide annotation of gene regulatory elements linked to cell fitnessSemantic proximity Read ↗

crisprQTL mapping as a genome-wide association framework for cellular genetic screensSemantic proximity Read ↗

Comprehensive analysis and accurate quantification of unintended large gene modifications induced by CRISPR-Cas9 gene editingSemantic proximity Read ↗

Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-Seq+Semantic proximity Read ↗

Convergent mutations in tissue-specific regulatory regions reveal novel cancer driversSemantic proximity Read ↗

CRISPR/Cas9 deletions induce adverse on-target genomic effects leading to functional DNA in human cellsSemantic proximity Read ↗

A new class of temporarily phenotypic enhancers identified by CRISPR/Cas9-mediated genetic screening.Semantic proximity Read ↗

CRISPR Interference (CRISPRi) and CRISPR Activation (CRISPRa) to Explore the Oncogenic lncRNA Network.Semantic proximity Read ↗

CRISPR Screens Provide a Comprehensive Assessment of Cancer Vulnerabilities but Generate False-Positive Hits for Highly Amplified Genomic Regions.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.