Article

Coupled single-cell CRISPR screening and epigenomic profiling reveals causal gene regulatory networks

2018-09-13

Abstract excerpt

<h4>Summary</h4> Here we present Perturb-ATAC, a method which combines multiplexed CRISPR interference or knockout with genome-wide chromatin accessibility profiling in single cells, based on the simultaneous detection of CRISPR guide RNAs and open chromatin sites by assay of transposase-accessible chromatin with sequencing (ATAC-seq). We applied Perturb-ATAC to transcription factors (TFs), chromatin-modifying fa...

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Literature Corpus work: 0c813d69-fd8f-566b-a574-f7e9f91934e5
DOI: 10.1101/414870

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