Article

CRISPR-Cas9-mediated genome engineering exaggerates genomic deletion at 10q23.31 including the <i>PTEN</i> gene locus mimicking cancer profiles

2023-04-06

Abstract excerpt

<h4>ABSTRACT</h4> The CRISPR-Cas9 system is a powerful tool for studying gene functions and has tremendous potential for disease treatment. However, precise genome editing requires thorough assessments to minimize unintended on- and off-target effects. Here, we report an unexpected deletion of a 287 kb region on Chromosome 10 (10q23.31) in chronic myelogenous leukemia HAP1 cells, which are frequently used in CRIS...

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Literature Corpus work: a718eb19-a8e9-5bc7-9179-d5fb8aa5ab60
DOI: 10.1101/2023.04.05.535680

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