Article

CRISPR gene and transcriptome engineering (CRISPRgate) improves loss-of-function genetic screening approaches

2024-05-14

Abstract excerpt

The CRISPR/Cas9 technology has revolutionized genotype-to-phenotype assignments through large-scale loss-of-function (LOF) screens. However, limitations like editing inefficiencies and unperturbed genes cause significant noise in data collection. To address this, we introduce CRISPR Gene and Transcriptome Engineering (CRISPRgate), which uses two specific sgRNAs to simultaneously repress and cleave the target gene...

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Literature Corpus work: 06b32410-3f35-581f-9fc3-276d67151f94
DOI: 10.1101/2024.05.14.594135

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