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Improved short nascent strand sequencing (iSNS-seq) enhances DNA replication origin detection and reduces non-origin biases

2025-12-24

Abstract excerpt

Identifying DNA replication origins in human and other metazoan genomes has been challenging, as highlighted by the fact that various methods for mapping them have produced conflicting results. A popular method, short nascent strand sequencing (SNS-seq), enriches newly replicated short single-stranded DNA by size selection and λ -exonuclease ( λ -exo) digestion of parental DNA. Surprisingly, SNS-seq has never be...

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Literature Corpus work: b5278c4a-37e5-591e-8652-ed5acce73f92
DOI: 10.64898/2025.12.22.696044

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