Most human DNA replication initiation is dispersed throughout the genome with only a minority within previously identified initiation zones

Abstract excerpt

BACKGROUND: The identification of sites of DNA replication initiation in mammalian cells has been challenging. Here, we present unbiased detection of replication initiation events in human cells using BrdU incorporation and single-molecule nanopore sequencing. RESULTS: Increases in BrdU incorporation allow us to measure DNA replication dynamics, including identification of replication initiation, fork direction,...

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